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Image Search Results
Journal: Brain pathology (Zurich, Switzerland)
Article Title: Proteomic profiling of the epileptic dentate gyrus.
doi: 10.1111/j.1750-3639.2010.00414.x
Figure Lengend Snippet: Figure 3. Schematic overview of the epileptogenesis paradigm and the tissue isolation and protein profiling methods. The isolated dentate gyrus (DG) was subjected to 2-D electrophoresis and all gels were sequentially stained with Pro-Q DPS, SYPRO Ruby and Coomassie Blue dye. PDQuest software was used to match spots and analyze expression patterns. Selected protein spots were identified by LC-MS/MS and verified using immunohistochemical and Western blotting approaches. The representative brain section was stained with cresyl violet: CA3 and CA1 cell layers are noted.
Article Snippet: All gels were scanned using a Bio-Rad VersaDoc imaging system, and images of the same gel scanned before and after poststaining were compared and processed using
Techniques: Isolation, Electrophoresis, Staining, Software, Expressing, Liquid Chromatography with Mass Spectroscopy, Immunohistochemical staining, Western Blot